Finds from prehistoric sites across Macedonia are present. The exhibition begins at an era prior to the appearance of modern humans, through the cast of the skull of the Ouranopithecus Macedoniensis, an early hominid, and moves on to the Palaeolithic era with the cast of the famous Petralona Skull.

The peaceful revolution of the Neolithic era is presented next, along with the crucial social transformations of the Bronze Age, through relevant thematic units.

The exhibits refer to the prehistoric peoples' survival tactics (hunting, fishing, farming, herding), as well as practices that followed the course of development of prehistoric communities, such as surplus storage, vessel and tool making technologies, metallurgy and trade.

The artefacts on display deal with the subsistence practices of prehistoric people (hunting, fishing, farming, herding) as well as other practices that developed alongside prehistoric communities, such as surplus storage, pot-making technology, metallurgy and exchange.

There are also thematic units dealing with issues of social beliefs and ideological concerns, such as appearance and adornment, social inequalities and the treatment of the body after death.