Notable for its cultural and temporal range and artistic quality, the Spanish Colonial collection of the Denver Art Museum is the most comprehensive collection in the United States and one of the best in the world.

Spanning three and a half centuries (c. 1492-1850), the collection of over 3,000 objects represents the diverse cultures and geographic areas of Latin America including Mexico, Guatemala, Panama, Colombia, Venezuela, Ecuador, Bolivia, Peru, Argentine, Chile, and the southwestern United States.